Interleukin-8 and neutrophil function in rheumatoid arthritis and seronegative polyarthritis

Abstract EN

Methodology : the relationships between interleukin–8 (IL-8) and neutrophil turnover in synovial fluid (SF) as measured with cytosine deaminized (CD) and Sf metabolites were studied in 36 patients, 20 rheumatoid arthritis (RA), median age and disease duration 62.5 and 14.5 years, respectively and 16 seronegative polyarthritis (SNP), median age and disease duration 34, and 4 years, respectively.

SF samples and blood samples were aspirated.

IL-8 Levels (measure with ELISA) were significantly elevated in RA as compared to SNP (p < 0.05).

Results : SF IL-8 levels correlated strongly with CD, lactase-glucose, and the lactase to glucose ratio, these parameters also showed some association with the measures of disease activity (p < 0.05).

These results suggest that factors responsible for neutrophil accumulation (probably IL-8) are present in SF in R.A patients, and these coincide with products of their activation (CD) The degree of neutrophil turnover is linked to the anaerobic metabolism of the synovial activity, interleukin– 8 (IL-8) a neutrophil activating peptide is produced by a number of cell types that become introduced in the early stage of tissue damage (Baggiolini et al and Stricter et al) in chronic arthritis's these include lymphocytes, monocytes and synovial fluid endothelial cells, the main stimuli for interleukin–8 (IL-8) production are tumor necrosis factor (TNF) and (1L-1) (1L-8) is the specific cytokine that up regulates neutrophil B2 integrins and induces neutrophil transmigration to inflammatory sites via a chemotactic gradient (Huber et al and Roberts et al).

Conclusion : IL-8 exerts its effects through a wide range of biological activities the possible end effect of IL-8 on neutrotrophils is the production of degenerative enzymes by degranulation and production of reactive oxygen species (ROS) (Daniels et al) through the action of membrane bound oxidase (Daniels RH, et al) IL-8 has been implicated in the pathogenesis of RA and other chronic inflammatory arthritis's (Seits et al and Beaulieu et al) cytosine deaminized (CD) has been shown to be related to disease activity in RA and SLE (Thompson, et al.

and Keith, et el.) its relationship in SNP is less well established (Hell well, et al) the aims of this study was to investigate whether serum and synovial fluid IL-8 levels were related to neutrophil turnover or to disease activity in RA and seronegative polyarthritis (SNP) (Jones, et al).