Effect of bovine viral diarrhea virus infection on DNA integrity and interferon-gamma (IFN-T)‎ release in buffalo macrophage

Abstract EN

The present study aimed to investigate the effectiveness of a national animal disease laboratory (NADL) strain as cytopathic bovine viral diarrhea virus (cp- BVDV) infection on DNA integrity of blood derived buffalo macrophages (M#) and Interferon-Gamma (IFN-y) release in vitro.

The virus (106, 4 TCID50) infect the matured M^, cells in one column of inoculated M# as well as non-infected M# were scraped daily and collected for DNA damage using Comet assay.

The level of IFN-y was determined too.

Comet microphotograph of inoculated M& along the 4 days tested period showed, balloon shaped tail with different percent of DNA damage.

The DNA damage is beginning at 24 h post inoculation (PI) and reached to the maximum at 72-96 h PI as compared to the negative control.

A qualitative measurement of IFN-y in the supernatant of inoculated M# as compared with the negative control revealed gradually increased of IFN-y secretion throughout tested period that beginning at the 2nd day PI and reach to the plateau out through the 3rd and 4th day PI.

In conclusion, BVDV has suppressive effects on immune cells as demonstrated in vitro that damage of macrophage.