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Immunohistochemical and molecular study of p16ink4a expression in pituitary adenoma
Joint Authors
Abd al-Munim, Hanan M.
Abd al-Rahim, Daliyah M.
Source
Journal of the Egyptian National Cancer Institute
Issue
Vol. 21, Issue 4 (31 Dec. 2009), pp.351-360, 10 p.
Publisher
Cairo University National Cancer Institute
Publication Date
2009-12-31
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Topics
Abstract EN
Background and Objectives : pituitary adenomas comprise 10-25 % of primary intracranial tumors.
The molecular mechanisms underlying their development and progression have not yet been clearly defined.
P16INK4A is frequently disrupted in human tumors including pituitary adenomas.
Our aim was to evaluate the expression of P16 protein expression and its relation with gene methylation in pituitary adenoma development and progression.
Material and Methods : Immunohistochemistry was performed on 34 formalin fixed paraffin embedded specimens of pituitary adenoma.
The p16INK4A gene methylation status was screened using an enzyme restricted polymerase chain reaction.
Results : Loss of p16 protein expression occurred in 43 / 19 (55.9 %) and P16INK4A methylation was detected in 14 / 34 (41.2 %) of tumor samples.
Both p16INK4A methylation and lack of p16 immunoreactivity were significantly related to larger tumor size and increased grade.
Patients with either p16-negative or methylated tumors were significantly older than patients with p16-positive or methylated tumors.
A significant positive correlation between loss of p16 protein expression and p16INK4A gene methylation was found.
Conclusion : these data suggest that p16 down regulation is a common event in pituitary adenoma and that its alteration might play an important role in genesis, growth progression, and biological behavior of pituitary adenomas.
Our findings could imply that hyper methylation of the p16INK4A gene; represents the major target and perhaps a required epigenetic event in human pituitary tumor genesis.
American Psychological Association (APA)
Abd al-Munim, Hanan M.& Abd al-Rahim, Daliyah M.. 2009. Immunohistochemical and molecular study of p16ink4a expression in pituitary adenoma. Journal of the Egyptian National Cancer Institute،Vol. 21, no. 4, pp.351-360.
https://search.emarefa.net/detail/BIM-273922
Modern Language Association (MLA)
Abd al-Munim, Hanan M.& Abd al-Rahim, Daliyah M.. Immunohistochemical and molecular study of p16ink4a expression in pituitary adenoma. Journal of the Egyptian National Cancer Institute Vol. 21, no. 4 (Dec. 2009), pp.351-360.
https://search.emarefa.net/detail/BIM-273922
American Medical Association (AMA)
Abd al-Munim, Hanan M.& Abd al-Rahim, Daliyah M.. Immunohistochemical and molecular study of p16ink4a expression in pituitary adenoma. Journal of the Egyptian National Cancer Institute. 2009. Vol. 21, no. 4, pp.351-360.
https://search.emarefa.net/detail/BIM-273922
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 358-360
Record ID
BIM-273922