Assessment of hepatic regeneration after partial hepatectomy in albino rats with induced bone marrow suppression

Abstract EN

Background : The liver has a remarkable capacity to regenerate after injury.

Within a week after partial hepatectomy (PH) hepatic mass is back essentially to what it was prior to surgery.

Hematopoietic stem cells (HSCs) may contribute in the regeneration and the renewal capacity of hepatocytes.

Purpose : To investigate the origin of hepatocytes in liver regeneration whether from resident hepatic stem cells or from circulated HSCs after suppression of HSCs from bone marrow by Benzene Methods : This experimental study included 24 adult male albino rats.

The studied animals were divided into 4 groups; first group (control group) was included 6 normal rats.

No intervention was carried out to this group of rates.

Second group (PH) group) was included 6 rats in which 70% PH was done.

Third group (Benzene group) was included 6 rats of in which bone marrow (BM) suppression was carried out by Benzene injection.

Ten injections were performed subcutaneously in a period of 3 weeks for induction of BM suppression.

Fourth group (Benzene + PH group) was included 6 rats in which BM suppression was carried out as 3rd group.

Seventy percent PH was then done to them.

Cytological changes during regeneration were assessed in all groups by ; the number of binucleate cells and the restored number of hepatocytes.

Mitotic index was performed in the second and the fourth groups.

The percent of regeneration was also calculated.

Immunohistochemistry technique was used for detection of CD34+ cells markers in liver tissues by using anti CD34+ cells antibodies (this technique is done in group 4 only).

Results : Our results found that mean weights of rats and assumed liver weights, there were no statistical significant differences between the studied groups.

PH group had shown higher regeneration rate than Benzene + PH group ; the former showed a mean loss of 19% (81 ± 2.1% of regeneration) of their original weight by the end of the first week, and the latter a mean loss of 30% (70 ± 0.7% of regeneration).

Number of binucleate cells there was a significant difference in benzene treated group compared to control group (p < 0.05).

Mitotic index there was significant higher mitotic index in partially hepatectomized after bone marrow suppression by benzene than partially hepatectomized group (p < 0.05).

We had used CD34 (CBR-E8) antibody in our study to detect CD34 antigen marker of mice origin Conclusions : It is indicated that the hepatocytes can regenerate the liver and restore its original size.

Hematopoietic stem cells may habitat the endothelial cells in the liver.

Their role in hepatic regeneration doesn't appear early after partial hepatectomy it mobilized from bone marrow to the damaged liver relay in the liver tissues for long time (> 7 days) then differentiate into non parynchematous liver tissues.