Influence of culture conditions on cellulase production by streptomyces sp. (strain j2)‎

Abstract EN

The purpose of this study was to determine the influence of growth conditions and medium composition on the cellulose enzyme production by Streptomyces sp.

Production of cellulose enzyme by a Streptomyces strain (J2) was detected on cellulose agar (CA) medium after 4 days of incubation at 28 22°C that exhibited a clear zone of mm around the colony.

Cellulose production was assayed by measuring the amount of glucose liberated in μmol / ml / min by using the dinitrosalicylic acid assay method.

The highest crude enzyme activity (432 U / l) was observed after 3 days of incubation at pH 7 and 60 °C in a medium that was supplemented with 0.5 % glucose, 0.2 % starch, and 0.2 % NH4CL.

However, enzyme production and activity were strongly decreased at 45°C and acidic pH.

Enzyme production and activity were also inhibited when Streptomyces strain (J2) was grown in CMC broth supplemented with arabinose and yeast extract as a sole carbon and nitrogen source, respectively.