Comparative studies between indirect hemagglutination (IHA) test and enzyme linked immunosorbent assays (ELISA) test for detection of serum antibody levels against infectious bursal disease (IBD) virus in commercial broilers
Other Title(s)
مقارنة بين اختبار الاليزا و اختبار تخثر الدم الغير مباشر لتحديد مستوى الأجسام المناعية ضد فيروس مرض التهاب غدة فابريشيوس المعدي في قطعان التسمين
Joint Authors
Dahshan, al-Husayni Muhammad
Husayn, Ahmad Mustafa
Source
Assiut Veterinary Medical Journal
Issue
Vol. 57, Issue 131 (31 Oct. 2011), pp.390-401, 12 p.
Publisher
Assiut University Faculty of Veterinary Medicine
Publication Date
2011-10-31
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Topics
Abstract EN
This study was done to compare between IHA test and ELISA test as a serological test for detection of serum antibody levels against Infectious Bursal Disease Virus (IBDV) in Commercial Broilers, 300 Serum samples were collected from different commercial broiler farms and slaughter Shops from in and around Beni-Suef Governorate.
These samples were divided into three groups according to the age of the birds i.e.
0-3 weeks (100 samples), 3-6 weeks (l00 samples) and 6-8 weeks (100 samples).
ELISA and Indirect Haemagglutination (IHA) test were used to titrate the serum antibodies against infectious bursal disease virus.
Hyper immune serum was raised in laboratory rabbits against IBDV vaccine (D-78).
It was used to identify and confirm the IBD virus from bursal tissues isolated from the IBD affected flocks using the qualitative tests i.e.
agar gel precipitation suspension was prepared and used to determine the antibody titer.
Results indicated that out of 300 test serum samples 195 (65 %) were positive.
The maximum positive samples belonged to 6-8 weeks of age (89 %) followed by 3-6 weeks (73 %) and 0-3 weeks (33 %), respectively while the result of ELISA test of high and low titers were 18 % and 82 % at 0-3 week of age then at age 3-6 weeks the high titers were 35 % and low titers were 65 % and finally high ELISA titers 69 % and low titers 3 l % at age 6-8 weeks.
American Psychological Association (APA)
Dahshan, al-Husayni Muhammad& Husayn, Ahmad Mustafa. 2011. Comparative studies between indirect hemagglutination (IHA) test and enzyme linked immunosorbent assays (ELISA) test for detection of serum antibody levels against infectious bursal disease (IBD) virus in commercial broilers. Assiut Veterinary Medical Journal،Vol. 57, no. 131, pp.390-401.
https://search.emarefa.net/detail/BIM-274648
Modern Language Association (MLA)
Dahshan, al-Husayni Muhammad& Husayn, Ahmad Mustafa. Comparative studies between indirect hemagglutination (IHA) test and enzyme linked immunosorbent assays (ELISA) test for detection of serum antibody levels against infectious bursal disease (IBD) virus in commercial broilers. Assiut Veterinary Medical Journal Vol. 57, no. 131 (Oct. 2011), pp.390-401.
https://search.emarefa.net/detail/BIM-274648
American Medical Association (AMA)
Dahshan, al-Husayni Muhammad& Husayn, Ahmad Mustafa. Comparative studies between indirect hemagglutination (IHA) test and enzyme linked immunosorbent assays (ELISA) test for detection of serum antibody levels against infectious bursal disease (IBD) virus in commercial broilers. Assiut Veterinary Medical Journal. 2011. Vol. 57, no. 131, pp.390-401.
https://search.emarefa.net/detail/BIM-274648
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 399-401
Record ID
BIM-274648