Genetic identification of some egyptian vicia faba l. Cultwars and theht relation to orobanche crenata l. Tolerance

Abstract EN

Ten Egyptian faba bean cultivars were assessed for Orobanche tolerance in a field trial during the winter season 2002-2003.

Two traits related to Orobanche infection were estimated i.e., number of spikes/plant and weight of Orobanche stems/plant.

Statistical analysis revealed differences among the studied cultivars in relation to Orobanche tolerance/susceptibility.

The results confirmed that Giza 843 and Misrl were the most tolerant cultivars, while Giza 643, Giza716 and Giza717 were the most susceptible ones.

SDS-PAGE and RAPD analysis were used to study the genetic relationships among the ten faba bean cultivars and to detect some molecular genetic markers for Orobanche tolerance /susceptibility.

Both SDS-PAGE and RAPD analysis revealed distinctive bands that successfully determined tolerant from susceptible bean cultivars.

Data analyses for bdth SDS-PAGE and RAPD-PCR were used to construct a dendrogram showing the genetic relationships among the faba bean cultivars and fitted well with their pedigrees.