Molecular genetics characterization of some gamma rays peanut mutants

Abstract EN

Local peanut cultivars; Giza 4 and Giza 5 were exposed to different doses (50, 100, 150, 200, 250 and 300 Gy) of Co6t) gamma rays source.

All irradiated materials were cultivated to give the Mi and M2 generations.

Then, high yielding mutants were selected from the M2 generation and cultivated separately to give the M3 generation.

Some yielding component traits were measured for the three generations (Mi, M2 and M3) and M3 families.

The M3 selected mutants (highest yielding mutants and lowest yielding mutants) were artificially ^Infected by Aspergillus flavus L spores (Aflatoxins - Bl and B2 groups - producer strain).

Aflatoxin concentrations were estimated and the analysis showed that the aflatoxin concentrations in seeds of the M3 selected mutants from Giza 4 cultivar were higher than those in seeds of the M3 mutants selected from Giza 5 cultivar.

However, the concentration of aflatoxin was increased when the yield (weight of seeds per plant trait) increased.

RAPD-PCR analysis using primers A19, A20, B01, B08, C13 and C18 were successfully differentiated these mutants from their controls and showed high polymorphism among the M3 families.

Key-words: peanut, Arachis Hypogaea L., RAPD-PCR, yield X^ component traits, mutants, gamma rays, irradiation,1 fungs, Aspergillus flavus L., aflatoxins.