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Albumin aggregates : hydrodynamic shape and physico-chemical properties
Joint Authors
Atmeh, Raghib F.
Arafah, Isam M.
al-Khatib, Maysun
Source
Issue
Vol. 2, Issue 2 (31 Oct. 2007), pp.169-182, 14 p.
Publisher
Yarmouk University Deanship of Research and Graduate Studies
Publication Date
2007-10-31
Country of Publication
Jordan
No. of Pages
14
Main Subjects
Topics
Abstract EN
Although albumin was known for more than a century, little is known about its tertiary structure.
At present two models are known to represent its molecular conformation : the heart shaped and the prolate ellipsoid.
Our results support the prolate ellipsoid model for albumin in slightly alkaline solution.
Albumin in solution exists as a monomer and higher aggregates.
We isolated the monomer, dimer, tetramer, and hexamer of bovine serum albumin by gradient polyacrylamide gel electrophoresis followed by electro elution.
The monomer was stable for 5 months at 8oC, whereas the dimer and tetramer dissociated into smaller forms within few hours after their separation but no higher aggregates were detected.
Albumin forms are surrounded by about three layers of water, with Stokes’ radii of 3.55, 4.34, 5.18, and 5.85 nm for monomer, dimer, tetramer, and hexamer, respectively.
The effective negative charge and density of surface charge (-esu.cm-2), respectively, were 14 and 4244 (monomer), 12.3 and 2495 (dimer), and 10.2 and 1452 (tetramer).
The translational diffusion coefficients were 5.88, 4.81, 4.03, and 3.57 cm2.s-1 for monomer, dimer, tetramer, and hexamer, respectively.
Other physico-chemical properties were calculated such as : frictional coefficient, sedimentation coefficient, electrophoretic mobility, volume of hydrated and anhydrate molecules.
American Psychological Association (APA)
Atmeh, Raghib F.& Arafah, Isam M.& al-Khatib, Maysun. 2007. Albumin aggregates : hydrodynamic shape and physico-chemical properties. Jordan Journal of Chemistry،Vol. 2, no. 2, pp.169-182.
https://search.emarefa.net/detail/BIM-276904
Modern Language Association (MLA)
Atmeh, Raghib F.…[et al.]. Albumin aggregates : hydrodynamic shape and physico-chemical properties. Jordan Journal of Chemistry Vol. 2, no. 2 (Oct. 2007), pp.169-182.
https://search.emarefa.net/detail/BIM-276904
American Medical Association (AMA)
Atmeh, Raghib F.& Arafah, Isam M.& al-Khatib, Maysun. Albumin aggregates : hydrodynamic shape and physico-chemical properties. Jordan Journal of Chemistry. 2007. Vol. 2, no. 2, pp.169-182.
https://search.emarefa.net/detail/BIM-276904
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 181-182
Record ID
BIM-276904