In vitro propagation of two lavandula species : lavandula angustifolia and lavandula latifolia L medica

Abstract EN

A In vitro propagation of Lavandula angustifolia and Lavandula latifolia was studied.

In vitro Cultures of Lavandula angustifolia and Lavandula latifolia were established by germinating seeds of each species on Murashique and Skoog (MS) medium supplemented with 0.5 mg/L Naphthaline Acetic Acid (NAA) incubated for three weeks in the dark at 28° C.

The best shoot multiplication rates (2.0) and highest proliferation of Lavandula angustifolia were obtained when internodal segments were subcultured on MS medium supplemented with 1.5 mg/L Kinetin and 0.05 mg/L NAA.

Shoots were rooted on MS medium supplemented with 0.4 mg/L of NAA or Indole Butyric Acid (IBA).

Plants were successfully transferred to soil after two weeks of acclimatization in the greenhouse.

Shoot multiplication rates and proliferation of Lavandula latifolia were best when MS medium was supplemented with 0.5 mg/L Benzyl Amino Purine (BAP), 0.05 mg/L NAA or with 1.0, 1.5 or 2.0 mg/L Kinetin and 0.05 mg/L NAA Shoots were rooted on MS supplemented with 0.3 mg/L NAA.