Some biochemical and haematological studies in brucellosis infected buffaloes, cows and sheep in El-Sharkia Governorate

Abstract EN

Blood samples from 351 buffaloes, 149 cows and 551 sheep were taken from a farm at New Salhia, (El-Sharkia Governorate) and examined for the incidence of brucellosis by the following serological tests, qualitative buffered acidified plate antigen (BAPAT), Rose Bengal Plate test (RBPT), the standard quantitative tube agglutination test (SAT) and the confirmatory quantitative complement fixation test (CFT).

Biochemical and hematological studies were done on 60 seropositive (20 from each species) as well as 60 seronegtaive animals (20 from each species) as control ones the biochemical analysis revealed a significant increased enzymatic activities for both, asparatate aminotransferase (AST) and alanine aminotransferase (ALT), and a significant increase in urea level of infected animals as compared with the control ones.

On the other hand, a significant decrease was recorded in total serum protein and glucose levels in all infected species.

Serum iron level showed a significant decrease in infected cows only.

Whereas, no changes were detected for both serum calcium and inorganic phosphorus levels in infected animals comparing with control ones.

Concerning hematological profile, we recorded a significant decrease in Hb and PCV % in all infected species accompanied with a general decrease in RBCs count, only of significant value in infected cows.

The WBCs count showed insignificant alterations in all infected animals compared with control ones.