Clinicoepidemiological studies and evaluation of pcr on diagnosis of lumpy skin disease outbreak, Egypt 2005

Abstract EN

In this study the last outbreak of lumpy skin disease (lsd) was investigated from june 2005 to april 2006 over two egyptian governorates (ismailia and damietta).

A total number of 954 (103 native and 851 mixed) cattle breed were examined clinically for lsd.

Different clinical forms of the disease were reported and ranged from acute, mild and in apparent forms.

Total morbidity and mortality rates were 50.5 % and 1.1 % among examined cattle respectively.

different field samples were collected (buffy coat 20, skin biopsy 15 and lymph nodes biopsy 5) for virus isolation and polymerase chain reaction technique (pcr) and the isolated virus was identified using virus neutralization test (vnt) and indirect fluorescent antibody technique (ifat), while 200 sera samples were collected from both diseased and contact healthy cattle for serological investigations.

The present study showed that the old age groups, male and native breeds were more resistant to infection.

the results of serological examinations revealed that the incidence of infection was 58 % and 49 % by serum neutralization test (snt), while by enzyme linked immune sorbent assay (elisa) were 62 %, and 55 % in Ismailia and Damietta governorates respectively.

Elisa test was more sensitive than snt in early detection of infection as it detect the infection by 7 day while snt by 10 days after fever.

The results of pcr test were more sensitive and rapid for detection of the virus as compared with isolation and identification technique when applied on blood and tissue samples therefore we can use it as rapid means for lsd diagnosis.