Angiogenesis in rheumatoid patients : clinical, radiological and histopathological study
Joint Authors
al-Husayni, Muna Jamal al-Din
Abd al-Nasir, Ula
al-Ganzouri, Amal Mustafa
al-Hifni, Abd al-Azim Muhammad
Muahmmad, Nermin H.
Ismail, Nifin T.
Talat, Muna
Source
Egyptian Rheumatology and Rehabilitation
Issue
Vol. 32, Issue 4 (31 Jul. 2005), pp.479-497, 19 p.
Publisher
The Egyptian Society for Rheumatology and Rehabilitation
Publication Date
2005-07-31
Country of Publication
Egypt
No. of Pages
19
Main Subjects
Topics
Abstract EN
Objective : To evaluate angiogenesis and cartilage destruction in rheumatoid arthritis (RA) patients.
This was performed using power Doppler ultrasonography (PDS) and detection of vascular endothelial growth factor (VEGF) in the serum and immunohistochemically.
Methodology : Eighteen Rheumatoid arthritis patients (group 1) and ten apparently healthy subjects (group 2) participated in this study.
Full history taking, thorough clinical examination and routine rheumatologic profile investigation were done.
Serum VEGF was measured in all patients and controls using ELISA technique.
The knee joints were examined with ultrasound and resistance index was measured with spectral ultrasonography.
Histological examination for synovial membranes was done with hematoxylin and eosin, factor VIII and VEGF with immunohistochemical staining.
Results : there was a highly significant difference between RA patients and controls as regard sVEGF (582.22 ± 84.89, 176 ± 20.55 pg / ml), histological score (36.94 ± 7.83, 2.72 ± 1.2), factor VIII (3.16 ± 0.618, 1.2 ± 0.41), VEGF staining (3.03 ± 0.89, 0.5 ± 0.32) and PDS (3.027 ± 0.58, 0.41 ± 0.24) (p < 0.001 in all parameters).
Also, there was a significant difference between patients and control as regard resistive index (RI) (0.72 ± 0.19, 1.07 ± 0.1) and synovial proliferation (3.055 ± 0.7, 0.25 ± 0.263) (p < 0.05 in both parameters).
RA patients were classified according to VEGF staining into low, moderate and intense staining.
There was a highly significant difference between patient subgroups regarding serum VEGF, synovial proliferation, PDS score (p < 0.001) and a significant difference regarding histopathological score, factor VIII and RI (p < 0.05 in all parameters).
There was a positive correlation between sVEGF and DAS score, synovial proliferation, ESR, PDS, VEGF staining and histological score, and negative correlation with RI.
Also, there was a positive correlation between VEGF staining and histological score, sVEGF, PDS, synovial proliferation and negative correlation with RI.
Conclusion : VEGF is a potent mediator of endothelial proliferation of angiogenesis, the expression of VEGF depending on the activity and plays a part of pathogenesis of RA and sinusitis.
PDS is a useful method demonstrating synovial vascularization and monitoring disease activity.
RI is an objective tool to estimate the degree of inflammation in RA.
American Psychological Association (APA)
al-Husayni, Muna Jamal al-Din& al-Ganzouri, Amal Mustafa& Abd al-Nasir, Ula& al-Hifni, Abd al-Azim Muhammad& Muahmmad, Nermin H.& Ismail, Nifin T.…[et al.]. 2005. Angiogenesis in rheumatoid patients : clinical, radiological and histopathological study. Egyptian Rheumatology and Rehabilitation،Vol. 32, no. 4, pp.479-497.
https://search.emarefa.net/detail/BIM-27840
Modern Language Association (MLA)
al-Husayni, Muna Jamal al-Din…[et al.]. Angiogenesis in rheumatoid patients : clinical, radiological and histopathological study. Egyptian Rheumatology and Rehabilitation Vol. 32, no. 4 (Jul. 2005), pp.479-497.
https://search.emarefa.net/detail/BIM-27840
American Medical Association (AMA)
al-Husayni, Muna Jamal al-Din& al-Ganzouri, Amal Mustafa& Abd al-Nasir, Ula& al-Hifni, Abd al-Azim Muhammad& Muahmmad, Nermin H.& Ismail, Nifin T.…[et al.]. Angiogenesis in rheumatoid patients : clinical, radiological and histopathological study. Egyptian Rheumatology and Rehabilitation. 2005. Vol. 32, no. 4, pp.479-497.
https://search.emarefa.net/detail/BIM-27840
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 493-497
Record ID
BIM-27840