Identification of theileria species in sheep and goats by the polymerase chain reaction (PCR)
Joint Authors
Radwan, I. G. H.
al-Kelesh, E. A.
Source
Kafr El-Sheikh Veterinary Medical Journal
Issue
Vol. 7, Issue 1 (31 May. 2009), pp.460-473, 14 p.
Publisher
Kafr El-Sheikh University Faculty of Veterinary Medicine
Publication Date
2009-05-31
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Topics
Abstract EN
This study was carried out to determine the rate of Theileria infection in sheep and goats and identify the species by polymerase chain reaction (PCR).
The prevalence of Theileria infection in sheep and goats was 33.75 % and 28 % respectively, by microscopical examination of blood smears from 80 sheep and 50 goats in Egypt.
Five positive samples for Theileria infection were subjected to PCR using Theileria species specific primer.
In addition, 5 negative samples for Theileria infection and apparently healthy were subjected to the same examination.
Seven out of 10 blood samples (5 sheep and 2 goats) were PCR positive using Theileria species specific primer at 1098 bp.
Three of those were PCR positive using T.
ovis specific primer at 520 bp.
T.
lestoquardi was detected in 3 samples by PCR at 230 bp.
Two apparently healthy animals were positive for Theileria species PCR but negative for both T.
lestoquardi and T.
ovis.
This indicated that, other nonpathogenic Theileria species may be present in Egypt.
Only one sheep sample was showed mixed infection of both T.
ovis and T.
lestoquardi.
American Psychological Association (APA)
Radwan, I. G. H.& al-Kelesh, E. A.. 2009. Identification of theileria species in sheep and goats by the polymerase chain reaction (PCR). Kafr El-Sheikh Veterinary Medical Journal،Vol. 7, no. 1, pp.460-473.
https://search.emarefa.net/detail/BIM-278456
Modern Language Association (MLA)
Radwan, I. G. H.& al-Kelesh, E. A.. Identification of theileria species in sheep and goats by the polymerase chain reaction (PCR). Kafr El-Sheikh Veterinary Medical Journal Vol. 7, no. 1 (2009), pp.460-473.
https://search.emarefa.net/detail/BIM-278456
American Medical Association (AMA)
Radwan, I. G. H.& al-Kelesh, E. A.. Identification of theileria species in sheep and goats by the polymerase chain reaction (PCR). Kafr El-Sheikh Veterinary Medical Journal. 2009. Vol. 7, no. 1, pp.460-473.
https://search.emarefa.net/detail/BIM-278456
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 471-473
Record ID
BIM-278456