Studies on the bacteria associated with respiratory disease of slaughtered sheep with special reference to mannheimia haemolytica

Abstract EN

This study aimed at investigating isolation, identification and confirmation of the isolated strains by biochemical tests and PCR, also biotyping of Mannheimia haemolytica strains isolated from sheep with pneumonia.

In addition, antibiotic susceptibility of the isolates were determined against a member of antimicrobial agents.

Mennheimia haemolytica was isolated in percentage of 52(20.8 %) from a total 250 samples, (120 pneumonic lungs, 90 Nasopharyngeal swabs, 40 Tonsils samples) were examined in the present study.

All samples were obtained from local state-run slaughterhouse.

A total of 52 Mannheimia haemolytica strains, were isolated and identified.

Out of 52 Mannheimia haemolytica strains, four random strains were chosen and confirmed by PCR and the result indicate that the PCR go in accordance with those of biochemical tests.

From 52 sheep isolates, 42 (80.8 %) and 10 (19.2 %) were biotyped as biotype A and biotype T, respectively.

Biotype A strains of Mnnheimia haemolytica isolated in this study is more frequently isolated from sheep lungs and utilized arabinose better than xylose and did not ferment trehalose.

Therefore, it may be suggested that fermentation of arabinose and trehalose are more reliable than that of xylose when performing biotyping.

In vitro antimicrobial susceptibility test results indicated that all Mannheimia haemolytica strains isolated were tested for their susceptibility to various antimicrobial agents using a disc diffusion method.

All 52 sheep isolates tested, were susceptible to enrofloxacin and danofloxacin.

In addition, isolates were found susceptible to gentamicin, streptomycin, and kanamycin with percentage 86.5 %, 76.9 %, and 69.2 % respectively.

All 52 isolates tested were resistant to clindamycin, oxytetracycline and sulfafurazole.

In addition, amoxicillin, oxacillin, ampicillin, tetracycline, erythromycin and penicillin G.

67.3 %,67.3 %, 59.6 %, 50 %,34.6 % and 30.8% respectively.