Quantitation of capripox purified viral proteins

Abstract EN

Sheep pox "SP", goat pox "GP" and lumpy skin disease "LSD" viruses were propagated and titrated individually in Vero or MDBK cell cultures and their tires were 5.6, 5.3 and 5.2 log10 TCID50 / ml respectively.

The viral proteins of these viruses were estimated by Bradford and Lowery techniques using spectrophotometer.

The first technique depends on standard curve while the second technique based on Lowery equation.

The quantity of viral proteins were 0.306, 1.440 and 2.074 mg / ml for SPV, GPV and LSDV respectively by using Lowery technique, while in Bradford technique, the viral proteins were 0.287, 1.297 and 1.986 mg / ml for SPV, GPV and LSDV respectively.

The results in Lowery technique were more sensitive and accurate than those obtained by Bradford technique.