Inactivation of foot and mouth disease viruses (types o1 and a1)‎ by using a combination of binary ethyleneamine and formaldehyde

Abstract EN

Foot and Mouth Disease Virus (FMDV) types (A / 1 Egypt 2006 and O1 Aga 1993) were isolated in Egypt and used in vaccine production.

When inactivated only with Binary ethylene mine (BEI) in concentration of 0.1M, the type A1 virus was completely inactivated after 15 hours also type O1 was completely inactivated after 14 hours at the same conditions when BEI was added only and the inactivation rates of 2 types ranged from 0.4–0.5 log10 / hour and Also results of CFT was 1 / 32 and 1 / 16 respectively for type O1 and 1 / 16 and 1 / 8 for type A / 1 before and after inactivation process also results of 146S content was ranged from 0.3 -0.4 ug / ml after and before inactivation of type O1 but for type A / 1 0.2 -0.3 ug / ml after and before inactivation process By using BEI-Formalin The results was for type A / 1 Egypt 2006 virus inactivated completely after 12 hours and for (type O1 / Aga 1993) after 10 hours at the same conditions ,and for Type A / 1 and O1 respectively.

The BEI-FA inactivation rate was ranged from 2.5 -3 log10 / hour and complement fixation test results not changed before and after inactivation for type A / 1 and O1 was 16 / 1 before and after inactivation for type A / 1 and for type O1 was23 / 1 The results of 146S content was 0.85 ug / ml for type O1 and for (A / 1) 0.7 ug / ml, before and after inactivation .It was clear that, the use of BEI-FA as in activator for type A/1 and O1 of FMDV.

Gave good and safe antigen content and reduce the time of inactivation.