Efficiency of binary ethel amine as inactivator for pigeon paramyxovirus-1

Abstract EN

Investigation studies on inactivated pigeon paramyxovirus-1 vaccine using binary ethylene mine were conducted.

The inactivation process of the local isolate of pigeon paramyxovirus-1 (PPMV-1) using the above inactivant revealed complete virus inactivation at 8, 6, 5 and 4 hours post treatment with inactivant concentrations of 0.0025, 0.003, 0.004 and 0.005 M respectively without detection of residual virus.

An inactivant concentration of 0.0025 M is recommended and no changes in viral antigenic properties were noticed.

Two experimental prepared batches of binary inactivated vaccine by two different adjuvants (oil or gel).

Oil adjuvant vaccine was superior than that prepared using alhydragel as adjuvant when they tested in pigeon as measured by its ability to produce higher log2 HI titre (29) four weeks post vaccination and persist for about five months as well as the highest protection percentage (95%).

On the other hand, pigeon vaccinated with alhydr-agel prepared vaccine had a protection percent (90%) and HI titre was (2.7.0) four weeks post vaccination.