Diagnostic and clinicobiochemical studies on sheep brucellosis and human contacts in sharkia governorate

Abstract EN

This study was carried out on one hundred sheep and fifty men at high risk in direct contact with these sheep from different localities in Sharkia Governorate.

These cases were subjected to serological tests for diagnosis of the disease.

These screening tests were Rose Bengal test (RBT) and Buffered Plate agglutination (BPAT).

seropositive samples were confirmed by Rivanol test (Ri-T) and tube agglutination test.

The results of examined revealed cases that thirty sheep (30%) and seven persons (14%) were seropositive.

Five serum samples were obtained from seropositive and another five serum samples of seronegative animals.

The procedures were repeated in seropositive and seronegative human.

They were subjected to biochemical examination.

Biochemical studies revealed a significant increase in sAST, sALT, sAP and serum globulin meanwhile a significant decrease in total protein, albumin, calcium and inorganic phosphorous of affected sheep and men compared with the healthy ones.

One blood sample was taken on sodium salt of EDTA from each case of the above examined cases.

They were subjected to hematological study.

The affected cases both animals and human revealed normocytic normochromic anemia associated with higher ESR, leukocytosis, neutrophilia and lymphopenia.

It could be concluded that brucellosis led to disturbance of general health condition of the host, so careful attention must be paid for early diagnosis of this zoontic disease followed by eradication of sero-reactor sheep; vaccination of non reactors and a therapeutic plan for the disease farm workers.