Electrophoretic profile of respiratory syncytial virus (rsv)‎ isolated from calves in Egypt

Abstract EN

Isolation and identification of bovine respiratory syncytial virus (BRSV) were described.

A total of 74 nasal swabs were collected from diseased calves with sudden onset of fever, lethargy, abdominal breathing, rhinitis, nasal discharge and cough.

Bronchiolitis may be associated with edema and emphysema and cases with sever bronchopneumonia.

Most commonly the infection occurs during the winter season when calves are housed in confined condition.

Viral diseases play an important role in meat quality.

The consumers were suffered from many problems if he consumed such infected meat.

The collected samples represented two Egyptian Governorates in which obtained 2 isolates from calves.

The identification of the virus was carried out by virus neutralization test and ELISA.

The present work describes the electrophoretic separation of BRSV in polyacrylamide gel electrophoresis in a glycine running buffer containg sodium dodecyl sulfate (SDS-PAGE) and staining with Coomassie blue.

The electrophoretic mobilities of RSV were compared with molecular weight marker.

The molecular weights of BRSV were detected at approximate range from 16.226 to 68.134 kilo Dalton (KDa.).

Bovine respiratory syncytial antigen was dotted on nitrocellulose membrane (NCM) against antisera applied on a absorbent paper strips.

The immune complexes of protein bands were detected with horseradish peroxidase – conjugated immunoglobulin’s.

A colour substrate was used to visualize the antigen-antibody complexes.

Control were consisted of positive and negative sera.