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Evaluation of the nitric oxide activity against blastocystis hominis in vitro and in vivo
Joint Authors
Eida, Umaymah M.
Eida, Amani Muhammad
al-Moamly, Amal M. Abd al-Rashid
Salim, Atiyyah M.
Husayn, Iman M.
Source
Journal of the Egyptian Society of Parasitology
Issue
Vol. 38, Issue 2 (31 Aug. 2008), pp.521-536, 16 p.
Publisher
The Egyptian Society of Parasitology
Publication Date
2008-08-31
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Topics
Abstract EN
The effect of exogenous nitric oxide (NO) on growth, viability and ultra-structural of B.
hominis was assessed in vitro by sodium nitrite (NaNO2) in 0.6 mM, 0.8 mM and 1 mM concentrations.
The viability of B.
hominis was identified using neutral red stain.
The role of NO as an endogenous oxidant was assessed by identifying its level in cecum tissue, ileum tissue, blood and stool elutes of mice infected with B.
hominis symptomatic human isolates using reactive nitrogen assay compared to control.
In vitro study revealed that NaNO2 inhibited the growth and decreased viability of B.
hominis with minimal lethal concentration dose 1 mM on the 4th day while, minimal effects were detected with 0.6 and 0.8 mM.
Transmission electron microscopy study proved that apoptotic-like features were observed in growing axenic culture of B.
hominis upon exposure to NaNO2.
These changes were not only found on the vacuolar (central body) form but also they were detected on granular, multi-vacuolar and cyst forms.
In vivo study proved that high levels of NO were found in infected mice compared to low changes in control group.
The high levels were in cecum tissue particularly.
The mean levels of NO among infected mice were 211.8 ± 20.7 µM in cecum, 90.4 ± 11.6 µM in ileum, 60.1 ± 4.7 µM in blood and 63.6 ± 7.3 µM in stool elutes while, the mean levels of NO in control mice were 70.2 ± 3.1 in cecum, 67.8 ± 4.7 µM in ileum, 30.9 ± 4.2 µM in blood and 28.1 ± 2.9 µM in stool elutes.
The differences were statistically highly significant.
NO-donor drugs pro-ved useful in treatment and increase the host resistance to B.
hominis.
American Psychological Association (APA)
Eida, Umaymah M.& Husayn, Iman M.& Eida, Amani Muhammad& al-Moamly, Amal M. Abd al-Rashid& Salim, Atiyyah M.. 2008. Evaluation of the nitric oxide activity against blastocystis hominis in vitro and in vivo. Journal of the Egyptian Society of Parasitology،Vol. 38, no. 2, pp.521-536.
https://search.emarefa.net/detail/BIM-279933
Modern Language Association (MLA)
Salim, Atiyyah M.…[et al.]. Evaluation of the nitric oxide activity against blastocystis hominis in vitro and in vivo. Journal of the Egyptian Society of Parasitology Vol. 38, no. 2 (Aug. 2008), pp.521-536.
https://search.emarefa.net/detail/BIM-279933
American Medical Association (AMA)
Eida, Umaymah M.& Husayn, Iman M.& Eida, Amani Muhammad& al-Moamly, Amal M. Abd al-Rashid& Salim, Atiyyah M.. Evaluation of the nitric oxide activity against blastocystis hominis in vitro and in vivo. Journal of the Egyptian Society of Parasitology. 2008. Vol. 38, no. 2, pp.521-536.
https://search.emarefa.net/detail/BIM-279933
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 532-536
Record ID
BIM-279933