Trichomonas vaginalis antigens in vaginal and urine specimens by immunochromatography, compared to culture and microscopy
Joint Authors
al-Moamly, Amal M. Abd al-Rashid
Rashid, Samir M.
Source
Journal of the Egyptian Society of Parasitology
Issue
Vol. 38, Issue 2 (31 Aug. 2008), pp.573-584, 12 p.
Publisher
The Egyptian Society of Parasitology
Publication Date
2008-08-31
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
This study evaluated the immunochromatographic (IC) capillary flow technology for detection of T.
vaginalis antigens in vaginal and urine specimens.
The IC antigen-detection test and microscopy wet smears were assessed against the InPouch® TV culture.
Vaginal-swa-bs and first-voided urine specimens were obtained from 348 sexually active symptomatic or asymptomatic, ≥ 16 years old women.
Nineteen samples (5 %) were positive by culture (95 %, CI 0.3-0.8).
The IC test (vaginal-specimens) was more sensitive than the wet smears.
IC sensitivity was 84% (95 %CI, 0.68-1.01), specificity 98% (95 %CI, 0.96-0.99), positive predictive value (PPV) 70 % (95 %CI, 0.51-0.88), negative predictive value (NPV) 99 % (95 %CI, 0.98-1), (positive likeli-hood ratio (+LR): 39.6, and negative likelihood ratio (–LR) 0.2.
The IC test using urine as a substrate had less performance than both microscopy and IC test of vaginal specimens.
Urine-IC sensitivity was 53 % (95 %CI, 0.30-0.75), specificity 99 % (95 % CI, 0.98-1.00), PPV 77% (95 %CI, 0.54-1), NPV 97% (95 %CI, 0.96-0.99), +LR : 57.7, and–LR: 0.5.
Sensitivity of vaginal smear was 68 % (95 %CI, 0.48-0.89), specificity 100 %, PPV 100 % (95 % CI, 1), NPV 98 % (95 % CI, 0.97-1), +LR >225, and–LR: 0.3.
T.
vaginitis was diagnosed in two wet uri-ne samples, but not in smears.
The IC antigen improved T.
vaginitis diagnosis, especially in screening, rapid, or point-of-care test, but in urine was less reliable than with vaginal smear.
American Psychological Association (APA)
al-Moamly, Amal M. Abd al-Rashid& Rashid, Samir M.. 2008. Trichomonas vaginalis antigens in vaginal and urine specimens by immunochromatography, compared to culture and microscopy. Journal of the Egyptian Society of Parasitology،Vol. 38, no. 2, pp.573-584.
https://search.emarefa.net/detail/BIM-279950
Modern Language Association (MLA)
al-Moamly, Amal M. Abd al-Rashid& Rashid, Samir M.. Trichomonas vaginalis antigens in vaginal and urine specimens by immunochromatography, compared to culture and microscopy. Journal of the Egyptian Society of Parasitology Vol. 38, no. 2 (Aug. 2008), pp.573-584.
https://search.emarefa.net/detail/BIM-279950
American Medical Association (AMA)
al-Moamly, Amal M. Abd al-Rashid& Rashid, Samir M.. Trichomonas vaginalis antigens in vaginal and urine specimens by immunochromatography, compared to culture and microscopy. Journal of the Egyptian Society of Parasitology. 2008. Vol. 38, no. 2, pp.573-584.
https://search.emarefa.net/detail/BIM-279950
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 580-584
Record ID
BIM-279950