Effect of artesunate on toxoplasma gondii : in vitro and in vivo studies
Author
Source
Journal of the Egyptian Society of Parasitology
Issue
Vol. 38, Issue 1 (30 Apr. 2008), pp.185-202, 18 p.
Publisher
The Egyptian Society of Parasitology
Publication Date
2008-04-30
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Topics
Abstract EN
The effect of Artesunate (As) on Toxoplasma gondii (T.
gondii) in vitro and in vivo was studied.
In vitro, tachyzoites of RH strain were exposed to As in a concentration of 2 µg/ml for 72 hours.
The assess-ment of As effect was carried out by studying the viability, infectivity and ultrastructure changes of treated tachyzoites by scanning electron microscope (SEM).
In the in vivo study, Swiss albino mice were infected intraperitoneally with tachyzoites of T.
gondii RH strain, and then orally treated with As in a dose of 200 mg / kg for five successive days.
The effect of As was evaluated by the mortality rate and survival time of the treated mice.
Parasite burden, viability, infectivity and ultrastructure changes of tachyzoites harvested from the peritoneal cavities' of infected treated mice as compared with infected non-treated control mice were also studied.
In vitro study demonstrated a signifi-cant reduction in viability and infectivity of tachyzoites exposed to As compared with untreated controls.
In vivo study, showed that treat-ment of infected mice with As induced a significant decrease in mort-ality rate and increase in survival time.
There was also a significant reduction in parasite burden in infected treated mice with significant reduction in viability and infectivity of tachyzoites harvested from them as compared with infected non-treated control.
SEM showed distortion in tachyzoites’ shape, peeling, erosions and discontinuity in areas of surface membrane of treated tachyzoites of both in vitro and in vivo studies.
So, As proved an effective and promising drug in treating acute toxoplasmosis.
American Psychological Association (APA)
al-Zawawi, Lubna A.. 2008. Effect of artesunate on toxoplasma gondii : in vitro and in vivo studies. Journal of the Egyptian Society of Parasitology،Vol. 38, no. 1, pp.185-202.
https://search.emarefa.net/detail/BIM-280298
Modern Language Association (MLA)
al-Zawawi, Lubna A.. Effect of artesunate on toxoplasma gondii : in vitro and in vivo studies. Journal of the Egyptian Society of Parasitology Vol. 38, no. 1 (Apr. 2008), pp.185-202.
https://search.emarefa.net/detail/BIM-280298
American Medical Association (AMA)
al-Zawawi, Lubna A.. Effect of artesunate on toxoplasma gondii : in vitro and in vivo studies. Journal of the Egyptian Society of Parasitology. 2008. Vol. 38, no. 1, pp.185-202.
https://search.emarefa.net/detail/BIM-280298
Data Type
Journal Articles
Language
English
Notes
Includes appendices : p. 201-202
Record ID
BIM-280298