Morphological and molecular identification of phaeoacremonium aleophilum associated with grapevines decline phenomenon in Duhok Governorate
Joint Authors
Abd Allah, Samir Khalaf
Halim, Raid A.
Jubrail, Jaladat M. S.
Source
Journal of Basrah Researches : Sciences
Issue
Vol. 37, Issue 4E (30 Sep. 2011), pp.1-8, 8 p.
Publisher
University of Basrah College of Education for Pure Sciences
Publication Date
2011-09-30
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Topics
Abstract EN
Decline symptoms on grapevine included plants that failed to thrive normal with reducing shoot growth and chloratic interveinal areas that later became necrotic.
In a cross section of grapevine arms, the internal wood tissue were frequently dark brown to black with a wedgeshaped necrotic sectors.
Phaeoacremonium aleophilum was isolated from infected tissues of declined plants in pure culture and identified on the basis of its morphological and cultural characteristics.
For accurate identification of P.
aleophilum the PCR technique was employed.
Ten isolates were selected from different locations.
These isolates were subjected to specific PCR assay.
The specific primers for P.
aleophilum were used to amplify the ITS region of nuclear ribosomal DNA (rDNA) containing ITS1, ITS2 and the intervening 5.8 rRNA genes.
PCR results obtained from Phaeoacremonium isolates indicated that only three isolates were related to P.
aleophilum.
The remaining isolates may represent different species of Phaeoacremonium.
P.
aleophilum is reported for the first time in Iraq.
American Psychological Association (APA)
Halim, Raid A.& Abd Allah, Samir Khalaf& Jubrail, Jaladat M. S.. 2011. Morphological and molecular identification of phaeoacremonium aleophilum associated with grapevines decline phenomenon in Duhok Governorate. Journal of Basrah Researches : Sciences،Vol. 37, no. 4E, pp.1-8.
https://search.emarefa.net/detail/BIM-285827
Modern Language Association (MLA)
Abd Allah, Samir Khalaf…[et al.]. Morphological and molecular identification of phaeoacremonium aleophilum associated with grapevines decline phenomenon in Duhok Governorate. Journal of Basrah Researches : Sciences Vol. 37, no. 4E (Sep. 2011), pp.1-8.
https://search.emarefa.net/detail/BIM-285827
American Medical Association (AMA)
Halim, Raid A.& Abd Allah, Samir Khalaf& Jubrail, Jaladat M. S.. Morphological and molecular identification of phaeoacremonium aleophilum associated with grapevines decline phenomenon in Duhok Governorate. Journal of Basrah Researches : Sciences. 2011. Vol. 37, no. 4E, pp.1-8.
https://search.emarefa.net/detail/BIM-285827
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 7-8
Record ID
BIM-285827