Antibacterial effect of lawsonia inerims linn. (henna)‎ using three types of leaf extract in vitro

Abstract EN

This study was done to investigate the antibacterial effect of the three types of Lawson inermis Linn (henna) leaf extracts (water, methanol and chloroform) in different concentrations (40, 80, 120) mg/ml against four strains of bacteria (Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli) in vitro using ager well diffusion method.

Water extract showed the highest antibacterial activity, followed by methanol extract, while the chloroform extract showed the lowest activity.

The maximum inhibition zone of water extract was observed against, Pseudomonas aeruginosa (25mm) in the concentration (120) mg/ml, while the minimum zone of inhibition (9mm) was in Bacillus subtilis in the same concentration.

The growth of all bacteria was inhibited by varying degrees with the increase of the extract's concentrations.

These results confirm the antibacterial activity of henna leaves and support the traditional use of the plant in therapy of bacterial infections.