The effect of sera of different animal species with oil adjuvant on immune response in white rats

Abstract EN

Blood samples of common carp (Cyprinus carpio L.) , chicken Gallus gallus , mice Mus musculus and human Homo sapines were collected sera were separated for each of them , then diluted into aratio 1 : 4 by using phosphate buffer saline(PH = 7.2) and mixed with oil adjuvant empty from microbial portion (sunflower oil).

The white rats Rattus rattus were immunized in constant immunization protocol for such sera and ten replicates for each treatment.

The blood samples were collected with anticoagulant and coagulant after terminate of immunization period in order to study the total and differential WBCs count, as well as to estimate the concentrations of total protein , Y-globulin, C3 and C4 of complement components, and immunoglobulin IgG in serum.

The results illustrate that part in sera of these animals lead to organization of immune response in rats into amplification , while the other part into suppression , also the study of cross- immunity among animals revealed similarity among sera of mice, chicken , and fish which is closely in their reactions with antisera of human obtained from primed rats during the appearance of partial precipitation results by using of double radial immunodiffusion assay , and this is indicate for similar of antigenic composition in spite of the difference in evolutionary pathway for each of them.