Isolation and identification of candida species and bacteria associated with orthodontic appliances experimental in vivo study

Abstract EN

This study employed the imprint culture technique to assess the effects of fixed and removal orthodontic appliances on oral carriage .specially the distribution of Candida species in group of adults during fixed and removable orthodontic appliance therapy.75 individuals, mean age=16.5 years, undergoing orthodontic treatment with fixed and removable orthodontic appliances.

the experimental group was selected from a larger sample of orthodontic patients who were clinically examined once to obtain baseline data before active treatment.18 males and 19 females were examined with fixed orthodontic appliances.

while, 17 males and 21 females were examined with removable appliances .Thereafter, the experimental group was examined three times after 3 and 6 months and over6 months follow-up period after insertion of orthodontic appliance.

The whole mouth plaque score was obtained, and the oral cavity was then sampled for Candida species and bacterial species by (imprint culture).

The results of this study showed that streptococcus species were present in higher percentages than other microorganisms, followed by Candida species, then staphylococcus, Pseudomonas and Lactobacillus species respectively.

Females showed higher bacterial growth compared with males, and higher mixed growth over single growth in female while male showed the reverse.

Number of bacterial growth after 6 months period was higher than 3 months period and both periods were higher than the period of over 6 months, with maximum bacterial growth of streptococcus species followed by Candida, then staphylococcus, Pseudomonas and Lactobacillus species respectively for all time intervals (except that Candida showed the same growth at 3 and 6 months period, and staphylococcus showed the same growth at 6 months and over 6 months period).fixed appliances showed more bacterial growth compared with removable appliance for all isolated microorganism types including Candida species, with maximum growth for streptococcus species .Results also revealed than maximum antibacterial resistance was for streptococcus species for the all four tested antibiotics, followed by Lactobacillus species, while the bacterial resistance for Candida, Staphylococcus and Pseudomonas species was very poor against the all tested antibiotics.