The activity of extracts of rosmarinus officinals and eucalyptus spathulata hook leaves on the growth of escherichia coli and candida albicans with estimation of the median lethal dose of both extracts in laboratory mice

Abstract EN

This study was conducted to investigate the activity of alcoholic extract of leaves of rosemary, Rosmarinus officinalis and Eucalyptus spathulata on the in vitro growth inhibition of Escherichia coli and Candid albicans by agar well diffusion method, with estimation of median lethal dose of both extracts.

Alcoholic extract of both leaves with three concentrations (100, 200 and 300 mg / ml) were prepared.

The three concentrations of both plants extracts showed high effect on the in vitro inhibition of growth of E.

coli.

The means of inhibition zone diameters were 13.2, 15.2 and 18.4 mm for R.

officinalis and 17.2, 18.2 and 20 mm for E.

spathulata.

There was moderate effect of the three concentrations of both extracts on the growth of C.

albicans, the means of inhibition zone diameters were10.2, 12.3 and 14.6 mm for R.

officinalis and 13.4, 15.2 and 16 mm for E.

spathulata.

The results of estimation of median lethal dose (LD50) indicated that there was no lethal effect of both leaves extracts in the laboratory mice, with the exception of the appearance of loss of appetite, increase respiratory and heart rates and ruffled hair without mortality at the doses ranged between 9000-20000 mg / kg body weight.