Effect of derum (juglan regia l. bark)‎ extracts on mutans streptococci in comparison to chlorhexidine gluconate : in vitro study

Abstract EN

Background : Derum is one of the natural "chewing sticks" obtained from the bark of walnut tree (Juglan regia L.) and used mainly by women in some countries as a tooth brush and as a dye for cosmetic purpose.

This study was conducted to test the effect of derum extracts on growth, adherence and acidogenicity of mutans streptococci in comparison to chlorhexidine gluconate 0.2 % as control positive and de-ionized water as control negative.

Materials and methods : Derum extracts were prepared by two methods (water and alcohol).

Stimulated saliva were collected from volunteers of dental students / University of Baghdad (21-23 years), from which Mutans Streptococci were isolated, purified and diagnosis according to morphological characteristic and biochemical test.

In vitro experiments were done to evaluate the effect of different types of derum extracts on growth, adherence and acidogenicity of mutans streptococci.

Results : Both types of derum extracts were effective in the inhibition growth of Mutans Streptococci and inhibition increased with the increasing concentrations.

Viability counts of mutans streptococci in comparison to control, at concentrations (20 % and 30 %) both types of derum extracts there were statistically significant reduction in the counts of Mutans Streptococci.

Both types of derum extracts (water and methanol) at concentrations (10 % and 20 %) were effective in prevent adherence and retardation of acid formation.

Conclusion : both types of derum extracts (water and methanol) were succeeded to prevent growth, adherence and acid production of Mutans Streptococci.