Evaluation of non surgical treatment of chronic periodontitis by assessment the enzymatic activity

Abstract EN

Background : Host responses to periodontal disease include the production of different enzymes that are released by stromal, epithelial or inflammatory cells into saliva and gingival reticular fluid.

There are important enzymes associated with cell injury and cell death like : lactate dehydrogenase (LDH), creatine kinase (CK), alkaline phosphates (ALP), Gama glutamyl transferase (GGT).

Changes in enzymatic activity reflect metabolic changes in the gingival and periodontium in inflammation.

To assess the difference in the activity of LDH, GGT and ALP enzymes in the saliva of male patients with chronic periodontitis before and after treatment, to test the effectiveness of treatment and to evaluate the correlation between these enzymes and the clinical parameters used for evaluation of periodontal tissues.

Materials and Methods : Measurements of plaque index (PLI), gingival index (GI), bleeding on probing (BOP), probing pocket depth (PPD) and clinical attachment level (CAL) were taken from ten subjects before treatment and one month after treatment ; Only male were included and saliva was collected for biochemical analysis of the enzymes alkaline phosphates ALP, lactate dehydrogenase LDH, and gamma glutamyle transferase GGT.

Results : Obtained results have shown that after conventional periodontal therapy, th activity of all salivary enzymes was significantly decreased.

However there was no correlation between the activities of these enzymes and the clinical periodontal parameters except between LDH with bleeding on probing and clinical attachment level.

Conclusion : Based on these results, it can be assumed that activity of these enzymes in saliva, as biochemical markers for periodontal tissue damage, may be useful in the diagnosis, prognosis and evaluation of periodontal therapy.