Efficacy of low level diode laser in skin wound healing : experimental study

Abstract EN

Background: Wound healing is a complicated, interactive, integrative process involving cellular and chemotactic activity, the release of chemical mediators and associated vascular response which includes number of phases: inflammatory phase, proliferative phase and remodeling phase.

Low level laser therapy can be more effective in the three overlapping phases of wound healing.

Biostimulation appears to have an effect on the cellular level, by increasing cellular function and stimulating various cells.

The aim of present study was to evaluate histologically the efficacy of low level diode laser on skin wound healing in animal model (histological study).

Material and methods: The experimental study was performed on seven healthy local bred rabbits.

Four incisions were done in each side at dorsal surface of each rabbit.

The wounds at the right side were irradiated by low level diode laser while the wounds at left side were left without irradiation as a control group.

Animals were divided into two groups according to laser parameters: 1- Group A includes three rabbits and the wounds were irradiated by 1.25W / cm2 power density, 20 sec.

exposure time.

2-Group B includes four rabbits and the wounds were irradiated by 1W/cm2 power density, 50 sec.

exposure time.

Histological specimens were taken at 2nd, 3rd, 5th and 7th day for microscopically examination concerning inflammatory cells infiltration and epithelial cell layer thickness.

Results : results show obvious reduction in inflammatory cell infiltration and more epithelization in laser treated wounds compared with control wounds.

Statistical analysis showed a significant difference between two groups.

Conclusion : low level diode laser (790-805nm) has beneficial effects in enhancement of soft tissue wound healing process histological.