The detection of human papilloma virus-16, in relation to p53 in oral squamous cell carcinoma by in situ hybridization

Abstract EN

Background : Squamous cell carcinoma of the oral cavity constituted a major health problem and can be a leading cause of death.

Several studies link high risk Human papillomavirus -16 to oral squamous cell carcinoma as an important etiological factor.

Several molecular markers have also been the subject of an intense research work, trying to clarify their role in oral carcinogenesis.

Of these, the cancer suppresser gene P53 has been extensively studied.

Aim of the study: 1.

to detect the association of the molecular markers Human papillomavirus -16, in relation to P53 in the oral Squamous cell carcinoma using in situ hybridization technique.

2.

To correlate Human papillomavirus -16 with clinical data (age, sex, risk factors grade and stage).

Material and Methods : tumor tissues from 33 patients with newly diagnosed as oral squamous cell carcinoma and who were surgically treated collected, formalin fixed and paraffin embedded.

Sections on charged slides were made from each tissue block and submitted to in situ hybridization technique utilizing Human papillomavirus -16, P53 cDNA probes.

Results: Human papillomavirus -16 was localized by in situ hybridization specifically within the nuclei of cancer cell it was detected in (27) (81.8 %) of 33 oral squamous cell carcinoma patients.

By using in situ hybridization P53 were detected in (93.9 %) cases.

There was no significant correlation between the Human papillomavirus -16 and P53 positivity.

Most of Human papillomavirus -16 positive patients were within the age group (50-59) years.

However, there was no significant correlation between Human papillomavirus -16 positive and age.

No significant correlation was found to sex and risk factors either.

Most of cases were moderately differentiated squamous cell carcinoma (79 %).

however no significant correlation was detected regarding histopathological grading or clinical staging with Human papillomavirus -16, and P53.

Conclusions: Being highly associated with Human papillomavirus -16 must be searched for in cancer tissues of such patients.