Evaluation of PCR-RFLP markers for maize (Zea mays L.)‎ genotypes

Abstract EN

PCR-RFLP analysis is a site targeted PCR where the non-arbitrary primers are used.

In this study, the primers used were selected among the available primers randomly.

Five pairs of chloroplast primers namely, rbcL, rpoB, psbC, N and A and one pair of ribosomal primers; IGS-rDNA, were used.

Among the six pairs of primers, three primers, rbcL, N and IGS-rDNA, were recommended to be used to analyse phylogeny of maize germplasm using the PCR-RFLP.

technique.

Polymorphism of PCR-RFLP technique is resulted from digestion of the targeted DNA sequence or PCR products using restriction enzymes.

Six restriction enzymes have been evalualted in this study, four of them BsuRI, Hind III, Pst I and TaqI, were found able to digest PCR products into smaller fragments, while two restriction enzymes, EcoRI and Bam HI, were unable to do so.

Generally, enzymes having four-base recongnition sites were found to have digested broader ranegs of PCR Products than those having six-base recognition sites.