Study the prevalence of helicobacter pylori infection by different diagnostic methods

Abstract EN

A total of 41 patients with gastro duodenal symptoms (show signs of inflammation with or without duodenal ulcer).

21 males (51.2%) and 20 female (48.8%) with an average age 0f (20 – 80( years old undergoing gastrointestinal endoscopy at Baghdad teaching hospital in internal disease clinical laboratory, between (February – June) 2009.

Biopsies specimen of antrum, gastric fundus, and duodenal bulb were examined by the following methods (rapid urease test, Giemsa stain section to detect bacteria, and Haematoxilin and Eosin stained section for pathological study which are considered the gold standard methods, sera or plasma from these patients were tested by immunochromotography (ICM),serological method for IgG antibodies to H.

pylori.

History picture are ( use of certain medication , tobacco , alcohol, and current infection are taken).

The results showed that the percentage of prevalence (positive results) were (83%) by histopathological method while it gave only (73%) by serological method and (66%) by rapid urease test, and the prevalence in males was more than in females (44%), (39%)respectively ,and also the prevalence increase with age (40 – 60) 14 out of 15, most patients show gastritis and duodenal ulcer, 25 (60%) by endoscopy diagnosis and 7 (17%) show malignant cancer ,while 9(22%) without any symptoms.

The sensitivity of urease test (82%) and specificity (88.1%) and by ICM sensitivity (86%) and specificity (67%) comparing with gold standard methods 100%.

The aim of this study is to compare the different diagnostic techniques of Helicobacter pylori infection by using invasive methods (histological examination of gastric and duodenal biopsies stained by Giemsa and Haematoxilin and Eosin methods, and rapid urease test which is considered the gold standard methods and non-invasive serological methods such as ICM rapid test , all these tests provide information about the incidence and prevalence of H.

pylori in population, diagnostic value for each test also the eradication of person.