Using tissue culture technique for the production of cardiac glycosides from roots of digitalis purpurea l plantlets (Var. excelsior mixed)‎

Abstract EN

The present study was performed using the tissue culture technique, where by roots formation was stimulated shoot tips of Digitalis purpurea L plant using auxins in this study, shoot tips (1cm length were excised from sterilized seedlings of Digitalis purpurea L.Var Excelsior mixed, then they were cultured on MS medium with the addition of one of the auxins IAA, IBA, NAA of different concentration (0.0, 0.05, 0.1, 0.5 and 1.0 mg / L After 45 days of starting the culture results showed that the treatment 0.5 mg / L of IBA had a high significant effect on the dry weight of roots formation and containing Digitoxin and Gitoxin whose quantities as a rate of (2.96 gm, 30.01 and 11.05 μg / gm dry weight respectively Also this treatment.

has given highest values with the other studied characteristics where the percent of the soluble sugars and starch and they are (2.48 %, 2.82%) respectively.