إنتاج نباتات الهيل Elettaria cardamomum Maton. بمرحلة واحدة من الأجنة المحفزة خارج الجسم الحي

Abstract EN

Stimulated embryos were excised from Elettaria cardamomum Maton.

Seeds.

The best of these were obtained from micropropagation MS + 2,4-D 5.0 mg / L for 20 days, and grown on the initiation medium MS+ BA 4.0 mg/L+NAA 1.0 mg / L.

They were differentiated to form plants in one–step regeneration with a number of 54 plants in an average of 18 plants/callus piece after 210 days of culture and in a response percentage of 75 %.Cardamom seed embryos, stimulated on the medium MS+2,4-D 25.0 mg/L for 20 days and subsequently cultured on initiation medium MS+BA 2.5 mg/L + NAA 1.0 mg / L.

They showed an ability to produce callus which were differentiated to plants in one–step regeneration earlier in comparision with a number of 45 plants, at an average of 15 plants / callus piece after 210 days of culture and in a response percentage of 60 %.This study succeeded in obtaining callus from immature embryos, removed from cardamom seeds, stimulated by the medium MS+2,4-D 20.0 mg / L and then grown on initiation medium MS+BA 1.5 mg / L +2,4-D 1.0 mg / L after 52 days post- culture in a percentage of 50 %.The best of average fresh weight of callus was 2.76 gm after 120days of culture.

The regenerated plants from tissue culture were successfully acclimatized after being hardened.