Electrophoretically mediated microanalysis based on azocoupling reaction for determination of phenols

Abstract EN

The capillary electro migration method is an important analytical technique known for its simplicity, sensitivity, and selectivity.

We have investigated electrophoretically mediated microanalysis (EMMA) in plug-plug and continuous formats for the determination of phenolic compounds.

The azocoupling reactions of phenol, 3-aminophenol, and resorcinol with 4- nitrophenyldiazonium fluoroborate were carried out in a capillary under electrophoretic conditions.

Procedures for determination of phenol, 3-aminophenol, and resorcinol were proposed and applied for analysis of commercial hair dyes.

The EMMA method based on the plug-plug format provided 100- to 1000-fold improvement in sensitivity in comparison with traditional CE.