P53 mutation in hcv-genotype-4 associated hepatocellular carcinoma in egyptian patientse
Joint Authors
Asad, Nansi Yusuf
Zekri, Abd al-Rahman N.
Bahnasi, Abir A.
Madbouly, Maha S.
al-Shihabi, Amal M.
Alam al-Din, Hana M.
Source
Journal of the Egyptian National Cancer Institute
Issue
Vol. 18, Issue 1 (31 Mar. 2006), pp.17-29, 13 p.
Publisher
Cairo University National Cancer Institute
Publication Date
2006-03-31
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Topics
Abstract EN
HCV-associated hepatocellular carcinoma (HCC) is a common neoplasm in Egypt where genotype-4 is prevalent.
In the present study the incidence and pattern of p53 mutations was assessed in relation to HCV-genotype- 4 in Egyptian HCC patients.
We investigated 25 HCV positive HCCs for p53 Mutations / overexpression in relation to HCV-NS3 by immunohistochemistry, SSCP and sequencing.
Genotyping was done using LiPA-II and TRUGENE 5’ NC’ sequencing kit.
Results were correlated to standard clinicopathologic prognostic factors for HCC.
Thirteen cases showed p53 overexpression, and 10 showed p53 mutation (13 mutations) by sequencing (72 % concordance).
The highest mutation rate was in exons 6 and 7 (30 %) followed by exons 5 and 8 (20 %).
Mutations included 3 transitions, 5 transversions, 3 deletions, and 2 insertions.
All exon 7 mutations were at codon 249 specific for AFB1 (AGG AGT, Arg Ser) and codon 248 specific for vinyl chloride contamination (CGG TGG, Arg Trp).
Other mutations reported are novel.
Immunostaining for HCV NS3 was detected in 19 cases independent of p53 mutation.
p53 aberrations were significantly associated with poor prognostic factors for HCC.
However, no specific pattern for p53 mutations was observed in HCV genotype 4-associated HCC and no significant relation between p53 mutations, HCV-NS3 expressions or any HCV sub-genotype-4 sequence.
American Psychological Association (APA)
Zekri, Abd al-Rahman N.& Bahnasi, Abir A.& Madbouly, Maha S.& Asad, Nansi Yusuf& al-Shihabi, Amal M.& Alam al-Din, Hana M.. 2006. P53 mutation in hcv-genotype-4 associated hepatocellular carcinoma in egyptian patientse. Journal of the Egyptian National Cancer Institute،Vol. 18, no. 1, pp.17-29.
https://search.emarefa.net/detail/BIM-29124
Modern Language Association (MLA)
Zekri, Abd al-Rahman N.…[et al.]. P53 mutation in hcv-genotype-4 associated hepatocellular carcinoma in egyptian patientse. Journal of the Egyptian National Cancer Institute Vol. 18, no. 1 (Mar. 2006), pp.17-29.
https://search.emarefa.net/detail/BIM-29124
American Medical Association (AMA)
Zekri, Abd al-Rahman N.& Bahnasi, Abir A.& Madbouly, Maha S.& Asad, Nansi Yusuf& al-Shihabi, Amal M.& Alam al-Din, Hana M.. P53 mutation in hcv-genotype-4 associated hepatocellular carcinoma in egyptian patientse. Journal of the Egyptian National Cancer Institute. 2006. Vol. 18, no. 1, pp.17-29.
https://search.emarefa.net/detail/BIM-29124
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 27-29
Record ID
BIM-29124