Chemical composition and biological activity of taxodium distichum l. Rich leaves growing in Egypt

Abstract EN

Objectives: To isolate flavonoids and volatile oil of Taxodium distichum leaves and evaluate the cytotoxic activity of the biflavonoid, amentoflavone against three types of tumor cell lines and evaluate the antiviral activity of the oil against Herpes simplex virus type 1 (HSV-1).

Methods: The dried powdered leaves of Taxodium distichum was extracted with 80% MeOH and then successfully extracted with pet.

ether (40-60 0C) and ethyl acetate.

Five flavonoids were isolated and identified by spectral analyses as, amentoflavone (1), rhamnetin (2), kaempferol-3-0-galactoside (3) luteolin-7-0-glucoside (4), quercetin-3-0-glucoside (5).

Cytotoxic activity of the isolated amentoflavone was performed against three types of tumor cell lines at a concentrations 1.00-10.00 ?g / ml using sulforhodamine B assay (SRB).The volatile oils of the fresh leaves was prepared by hydrodistillation method using Clevenger apparatus and the chemical composition of the oil was determined by GC/MS analysis .

The antiviral activity of the oil was tested against Herpes simplex virus type 1 (HSV-1).

Results: The tested compound exhibited a significant cytotoxic activity for U251, brain tumor cell lines (IC50 = 4.5 ?g / ml) while the IC50 was > 10 ?g/ml for both Hela and HEPG2 cell lines.

The major components of the volatile oil were terpinene-4-ol (38.37%) - pinene (14.51%), - terpinene (7.82%) and - terpineol (7.51%) and its antiviral activity against (HSV-1) was (74.68%) determined by plaque reduction assay method.

Conclusion: There is a potential cytotoxic activity of amentoflavone against brain tumor cell lines and significant antiviral activity of the volatile oils