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Assessment of tumor budding in colorectal carcinoma : correlation with b-catenin nuclear expression
Joint Authors
Source
Journal of the Egyptian National Cancer Institute
Issue
Vol. 23, Issue 1 (31 Mar. 2011), pp.1-9, 9 p.
Publisher
Cairo University National Cancer Institute
Publication Date
2011-03-31
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Topics
Abstract EN
Background : Tumor budding (TB) is showing increasing promise as a colorectal cancer (CRC) prognosticator that is independent of TNM staging.
B-Catenin is a component of the Wingless Want signaling pathway that is bound to membrane-associated E-cadherin and is essential for its correct position and function.
Methods : this study was designed to detect TB in 44 resected primary CRC cases and also to compare b-catenin expression in the tumor budding sites (TBS) and in the tumor center.
Tumor budding was assessed in both H&E and pankeratin immunostained sections.
Agreement between TB scoring using pan cytokeratin and H&E was tested.
Also, typing of the tumor margin and determination of degree of cytoplasmic pseudo-fragmentation was done.
Tumor budding, cytoplasmic pseudo fragments and b-catenin expression were related to known CRC prognosticators.
Results : Ten tumors (22.7 %) showed low grade (LG) budding and 34 tumors (77.3 %) showed high grade (HG) budding.
The 34 HG budding tumors were further subdivided into moderate and severe (n = 13, n = 21, respectively) budding cancers.
Twenty nine tumors (65.9 %) showed LG cytoplasmic pseudo fragments and 15 tumors (34.1 %) showed HG pseudo fragments.
Scoring of TB on H & E and pan keratin stained sections revealed moderate agreement (Kappa= .558; p= < .000).
A significant relation between TB and cytoplasmic pseudo fragments was observed (p= .009).
Both TB and cytoplasmic pseudo fragments did not significantly associate with clinic pathologic parameters.
Immunoreactivity of nuclear and cytoplasmic b-catenin was significantly higher at TBS compared to tumor center (p= .005, p= .000, respectively).
In opposition, membranous bcatenin expression was significantly higher in the tumor center than in TBS (p = .001).
Although, nuclear b-catenin accumulation at TBS was noted, yet, it did not relate significantly with both TB and cytoplasmic pseudo fragments around TBS (p = .649; p = .675, respectively).
Also, nuclear b-catenin immunoreactivity did not relate significantly with the various clinic pathological variables.
Conclusion : Pan keratin immunostaining facilitates typing of CRC invasive margin, and determination of the degree of TB and cytoplasmic pseudo-fragmentation.
Catenin expression differs significantly between tumor center and TBS in CRC.
Cut-offs for TB assessment should be unified and further studies are recommended to allow a better understanding of this process before establishing TB as a prognostic factor beyond the TNM staging in CRC.
American Psychological Association (APA)
al-Jundi, S.& al-Jundi, A.. 2011. Assessment of tumor budding in colorectal carcinoma : correlation with b-catenin nuclear expression. Journal of the Egyptian National Cancer Institute،Vol. 23, no. 1, pp.1-9.
https://search.emarefa.net/detail/BIM-293106
Modern Language Association (MLA)
al-Jundi, S.& al-Jundi, A.. Assessment of tumor budding in colorectal carcinoma : correlation with b-catenin nuclear expression. Journal of the Egyptian National Cancer Institute Vol. 23, no. 1 (Mar. 2011), pp.1-9.
https://search.emarefa.net/detail/BIM-293106
American Medical Association (AMA)
al-Jundi, S.& al-Jundi, A.. Assessment of tumor budding in colorectal carcinoma : correlation with b-catenin nuclear expression. Journal of the Egyptian National Cancer Institute. 2011. Vol. 23, no. 1, pp.1-9.
https://search.emarefa.net/detail/BIM-293106
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 8-9
Record ID
BIM-293106