Isolation, characterization and sensitivity test of organism found in bile obtained from sheep and cattle

Abstract EN

Objectives : The purpose of our study was to see if there were any pathogenic bacteria present in the raw bile of sheep and cattle used as an appetizer in some Sudanese food and to determine the sensitivity of the bacterial isolates to the common antibiotics used in the Sudan.

Specimens and Methods : This study was conducted in Omdurman, Sudan in the period from Nov 2003 to May 2005.

A total of 210 specimens from 108 sheep and 102 cattle were examined for the presence of pathogenic bacteria.

The isolates were identified by conventional methods.

Results : Thirty-five pathogenic bacteria were isolated from the bile of slaughtered clinically healthy sheep and cattle.

Seven isolates from sheep were classified as Escherichia coli, Pseudomonas stutzeri, Staphylococcus aureus and Acinetobacter lwoffii.

Twenty eight isolates from cattle were classified as Escherichia coli, Escherichia hermannii, and Escherichia vulneris, Proteus mirabilis, Pasteurella hemolytic var urea, Pseudomonas maltophilia, Pseudomonas cepacia, Pseudomonas alcaligenes, Bacillus cereus and Bacillus mycoides.

Antibiotic sensitivity tests were done for each isolate.

All isolates were found to be sensitive to pefloxacin (100 %), Ofloxacin (100 %), ciprofloxacin (97 %) and most of them were resistant to Cefotaxime (30.6 %).

All of the gram-negative isolates were sensitive to chloramphenicol, Piperacillin (96.8 %) ceftizoxime (90.3%), and amikacin (96.8 %) and gram positive isolates was sensitive to ciprofloxacin (100 %).

All of the gram positive isolate were resistant to co-trimoxazole, (100 %), Cefotaxime,(100 %) and Cephalexin (100 %).

Conclusion : This study shows that raw bile may serve as a potential source for infection to people who use it as food appetizer.