Determination of ascorbic acid by high performance liquid chromatography (H. RL. C.)‎ using photodiode array detector

Abstract EN

This paper describes a simple and rapid method for the determination of vitamin C using H.P.

L.

C.

A reversed phase C18 column was used to perform ion pairing chromatography, the ion pairing agent tri-n-butylamine and mobile phase 90:10 phosphate buffer (pH 4.

5) to methanol.

A diode array spectrophotometry detector facilitated method development allowing chromatograms to be obtained for a range of wavelengths.

A linear calibration curve was obtained in range 0-50 (jg / ml with correlation coefficient of 0.

999 and detection limit was 1.

5 fjg / ml and relative standard deviation was 1.

8 % for ten samples at 10fjg / ml.