Studying effect of ph on the antimycotic performance of some disinfectants by using quantitative suspension test

Abstract EN

The antimycotic effect of some disinfectants was comparatively studied under different pH gradients.

Reference strains of C.albicans, A.niger and T.mentagrophytes were used in the current study.

Quantitative suspension test was used with an initial inoculum of 1.0x107 CFU/mL from each organism.

After different exposure time (1; 2; 5; 10; 15; 30 and 60 min), 1.0 mL was taken and added to 9.0 mL of the neutralizer to give the required concentration and left for 10 min before spreading of 0.1mL on Sabouraud Dextrose agar medium.

The CFU/mL was recorded for each time point and the reduction of the viable count was recorded as log10 of the count.

The obtained results revealed that glutaraldehyde, formaldehyde, phenols and quaternary ammonium compounds are highly effective at alkaline pH.

The acid pH gradient of these compounds either fails to give the required result or needs a longer time of exposure.

On the other hand, sod.

hypochlorite and standard phenol were showed high antimycotic efficiencies under acidic pH than alkaline ones.

Using the alkalinizing and/or acidifying agents observed no direct toxic effect on the organism.

This indicates that the pH may modify the disinfectant performance, which should be put in our mind during disinfection.