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Contribution of Nitric Oxide and Epidermal Growth Factor Receptor in Anti-Metastatic Potential of Paclitaxel in Human Liver Cancer Cell (HebG2)
Joint Authors
Sayyid-Ahmad, Muhammad M.
Muhammad, Muhammad A.
Source
Journal of the Egyptian National Cancer Institute
Issue
Vol. 17, Issue 1 (31 Mar. 2005), pp.35-41, 7 p.
Publisher
Cairo University National Cancer Institute
Publication Date
2005-03-31
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Topics
Abstract EN
Background : Paclitaxel is a general antineoplastic drug used against different types of experimental and human tumors.
Several anti-cancer drugs have been shown to stimulate nitric oxide (NO) production, which has been shown to affect many aspects of tumor biology.
Objective : This study was initiated to determine if paclitaxel stimulates NO production in HebG2 cells, and if so, whether NO interferes with the metastatic potential of HebG2 cells and contributes to paclitaxel cytotoxicity.
In addition, we sought to determine the relationship between NO production and the expression of epidermal growth factor receptor (EGFR) and matrix metaloproteinases (MMPs) in HebG2 cells.
Materials and Methods : The effects of paclitaxel (0.1-1000nM) on surviving fraction, NO production and the expression of EGFR, MMP-2 and MMP-9 were studied in human liver cancer cells (HebG2).
Results : Paclitaxel resulted in a significant dosedependent decrease in the surviving fraction of HebG2 cells.
A 62 % and 86 % decrease in the surviving fraction was attained at 10nM and 100nM paclitaxel, respectively.
Paclitaxel produced a significant increase in NO production, starting from 1nM.
A 64 % and 111 % increase in NO production was attained after exposure to 10nM and 100 nM of paclitaxel, respectively.
In all of the HebG2 cells treated with paclitaxel (1.0-1000nM) mRNA specific for EGFR, MMP-2 and MMP-9 were undetectable.
However, untreated HebG2 cells and those treated with paclitaxel (0.1nM) expressed mRNA specific for these markers.
Conclusion : This study suggests that : (1) increased production of NO may contribute to paclitaxel’s cytotoxicity against HebG2 cells, (2) paclitaxel may inhibit tumor metastasis via inhibition of the expression of EGFR and MMPs and (3) an inverse correlation exisits between NO production and expression of EGFR and MMPs.
American Psychological Association (APA)
Sayyid-Ahmad, Muhammad M.& Muhammad, Muhammad A.. 2005. Contribution of Nitric Oxide and Epidermal Growth Factor Receptor in Anti-Metastatic Potential of Paclitaxel in Human Liver Cancer Cell (HebG2). Journal of the Egyptian National Cancer Institute،Vol. 17, no. 1, pp.35-41.
https://search.emarefa.net/detail/BIM-29661
Modern Language Association (MLA)
Sayyid-Ahmad, Muhammad M.& Muhammad, Muhammad A.. Contribution of Nitric Oxide and Epidermal Growth Factor Receptor in Anti-Metastatic Potential of Paclitaxel in Human Liver Cancer Cell (HebG2). Journal of the Egyptian National Cancer Institute Vol. 17, no. 1 (Mar. 2005), pp.35-41.
https://search.emarefa.net/detail/BIM-29661
American Medical Association (AMA)
Sayyid-Ahmad, Muhammad M.& Muhammad, Muhammad A.. Contribution of Nitric Oxide and Epidermal Growth Factor Receptor in Anti-Metastatic Potential of Paclitaxel in Human Liver Cancer Cell (HebG2). Journal of the Egyptian National Cancer Institute. 2005. Vol. 17, no. 1, pp.35-41.
https://search.emarefa.net/detail/BIM-29661
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical References: p. 39-41
Record ID
BIM-29661