Evaluation of crude anthrax protective antigen as an adjutant for brucella abortus vaccine in mice

Abstract EN

Anthrax crude protective antigen (PA) was used in this study as an adjutant with Brucella aborts S19 strain vaccine in a ratio of 1 : 1 to immunize mice in dose of 0.1 ml s / c in order to decrease side effects of S19 vaccine and also to enhance its immunogenicity.

Three groups of mice were immunized with the mixture (composed of anthrax PA + S19, S19 alone, and sterile PBS solution as a control.

They were compared concerning cellular and humeral immunity.

The group treated with the mixture was the best regarding skin test, the difference of thickness was 1.51 ± 0.103 mm after 24 h and 1.14 ± 0.103 mm and 0.89± 0.100 mm after 48 and 72 h, respectively.

As well as that group revealed the highest antibody titer, which reported 0.7153 ± 0.08 nm as compared with 0.3743 ± 0.06 nm in S19 group.

These findings were verified by studying the histopathologically changes.