Production, purification and characterization of protease by aspergillus flavus under solid state fermentation
Joint Authors
Muthulakshmi, Chinnasamy
Gomathi, Duraisamy
Kumar, Dugganaboyana Guru
Ravikumar, Ganesan
Kalaiselvi, Manokaran
Uma, Chandrasekar
Source
Jordan Journal of Biological Sciences
Issue
Vol. 4, Issue 3 (30 Sep. 2011), pp.137-148, 12 p.
Publisher
The Hashemite University Deanship of Academic Research and Graduate
Publication Date
2011-09-30
Country of Publication
Jordan
No. of Pages
12
Main Subjects
Abstract EN
Protease production under solid state fermentation (SSF) was investigated using isolated Aspergillus flavus.
Different agroindustrial waste products were evaluated to check the possibility of potential utilization of substrates in SSF for protease production by Aspergillus flavus using wheat bran as a substrate.
The results showed that the optimum conditions for maximum protease production were found to be 7th day of incubation at pH 5.0, temperature 30oC ; inoculum size 3% ; substrate concentration 3 % and 3 % KNO3 as nitrogen source.
The purified enzyme produced 5.8 fold with recovery of 3.2 % by DEAE-column chromatography and the molecular weight was estimated to be 46kDa by SDS-PAGE.
It has a Vmax value of 60.0 U / mg and Km value of 0.6 mg/ml at pH of 7.
The enzyme activity was found to be stable at 500 C and it was stimulated by metal ions like Cu2+ and Zn2+ and inhibited by Ca2+ and Mg 2+.
American Psychological Association (APA)
Muthulakshmi, Chinnasamy& Gomathi, Duraisamy& Kumar, Dugganaboyana Guru& Ravikumar, Ganesan& Kalaiselvi, Manokaran& Uma, Chandrasekar. 2011. Production, purification and characterization of protease by aspergillus flavus under solid state fermentation. Jordan Journal of Biological Sciences،Vol. 4, no. 3, pp.137-148.
https://search.emarefa.net/detail/BIM-297963
Modern Language Association (MLA)
Muthulakshmi, Chinnasamy…[et al.]. Production, purification and characterization of protease by aspergillus flavus under solid state fermentation. Jordan Journal of Biological Sciences Vol. 4, no. 3 (Sep. 2011), pp.137-148.
https://search.emarefa.net/detail/BIM-297963
American Medical Association (AMA)
Muthulakshmi, Chinnasamy& Gomathi, Duraisamy& Kumar, Dugganaboyana Guru& Ravikumar, Ganesan& Kalaiselvi, Manokaran& Uma, Chandrasekar. Production, purification and characterization of protease by aspergillus flavus under solid state fermentation. Jordan Journal of Biological Sciences. 2011. Vol. 4, no. 3, pp.137-148.
https://search.emarefa.net/detail/BIM-297963
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 147-148
Record ID
BIM-297963
