In-vitro susceptibility of infective keratitis causative bacteria to topical povidone iodine

Abstract EN

Purpose : To evaluate the in-vitro susceptibility of bacterial isolates from the cases of corneal ulcer to povidone iodine.

Setting : Department of ophthalmology, and clinical microbiology research center, Shiraz University of Medical Sciences, Shiraz, Iran.

Patients and Methods : The in-vitro susceptibility and Minimum Inhibitory Concentration (MIC) of the bacteria isolated from the cornea of 50 cases of bacterial corneal ulcer to povidone iodine was determined by broth dilution method.

Concentrations of 1%, 2%, 5% and 10% of povidone iodine were tested.

Results : Bacterial isolates included 50% S.epidermidis, 20% P.aerogenosa, 10% S.

aureus, 4% Acintobacter, 4% S.pneumoniae, 4% E-coli, 2% Proteous, 2% S.viridance, 2% Diphtherias and 2% Bacillous.

Povidone iodine bactericidal efficacy for gram positive bacteria was at least 82.36%, 91.2%, 100% and 100% in 1%, 2%, 5% and 10% concentrations after 5 minutes exposure time.

The bactericidal efficacy of this agent over the gram negative bacteria was at, least 62.5%, 87.5%, 100% and 100% in 1%, 2%, 5% and 10% concentrations after 5 minutes exposure time, respectively.

Conclusion : Povidone iodine was shown to have a broad spectrum invitro bactericidal efficacy in cases of bacterial keratitis.

If further in vivo studies confirm the efficacy of this agent over the bacterial keratitis, it would be suggested as a broad spectrum medication for the treatment of these cases.