Biomass production of saccharomyces cerevisiae ( baker.s yeast )‎ using the cactus cladodes extract as a culture medium

Other Title(s)

إنتاج خميرة الخبز باستخدام عصير سيقان التين الشوكي كوسط للنمو

Dissertant

Hammad, Majed Sh.

Thesis advisor

al-Bashiti, Tariq Abd al-Qadir
Zabut, Bakir Mahmud

University

Islamic University

Faculty

Faculty of Science

Department

Department of Biological Science

University Country

Palestine (Gaza Strip)

Degree

Master

Degree Date

2008

English Abstract

This study aims to evaluate Cactus opuntia cladodes (COC) extract as an alternative medium for producing Saccharomyces cerevisiae (baker syeast) (BY), and to optimize the environmental conditions of its growth (pH, temperature).

The study design was a comparative study.

COC were obtained from the boarders of a farm in Northern govern ate at the age of 1-2 years.

The crude COC extract was diluted to 50 % before using.

The reactivated BY sample used also diluted to 10-7.

Experiment was carried out on both surface and submerged COC extract cultures.

The control media were both potatoes dextrose agar and broth (PDA, PDB).

SPSS system was used to analyze the obtained data.

The results showed that the BY can grow and proliferate in COC extract (50% dilution) where the average specific growth rate (µ) was 0.21 h.-1 The optimum pH for yeast growth was about (4.0) and the optimum temperature for good specific growth rate was 30 0C.

The average specific growth rate of BY in PDB was 0.25 h-1 and the optimum pH and temperature were 4.0 and 30 0C, respectively.

It was found also that .0 g / L of yeast (dry weight) was produced in COC extract, while .0 g / L was produced in PDB after 72 hours growth.

It was found also that the total yeast dry weight that produced in COC extract was about 4 .3 g / L, while an equal quantity of PDB produced about 5 .1 g / L.

The study found also that 1000g fresh weight of COC can produce about 62.0 g of BY.

It can be concluded that COC extract could be a good medium for producing BY under restricted economic conditions.

Main Subjects

Biology

No. of Pages

59

Table of Contents

Abstract.

Table of contents.

Chapter 1 : Introduction.

Chapter 2 : literature review.

Chapter 3 : Materials and methods.

Chapter 4 : Results and discussion.

Chapter 5 : Conclusions and recommendations.

American Psychological Association (APA)

Hammad, Majed Sh.. (2008). Biomass production of saccharomyces cerevisiae ( baker.s yeast ) using the cactus cladodes extract as a culture medium. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-300308

Modern Language Association (MLA)

Hammad, Majed Sh.. Biomass production of saccharomyces cerevisiae ( baker.s yeast ) using the cactus cladodes extract as a culture medium. (Master's theses Theses and Dissertations Master). Islamic University. (2008).
https://search.emarefa.net/detail/BIM-300308

American Medical Association (AMA)

Hammad, Majed Sh.. (2008). Biomass production of saccharomyces cerevisiae ( baker.s yeast ) using the cactus cladodes extract as a culture medium. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-300308

Language

English

Data Type

Arab Theses

Record ID

BIM-300308