Genetic variation of 15 autosomal short tandem repeat (STR) loci in the Palestinian population of Gaza Strip
Other Title(s)
الاختلاف الجيني ل 15 موقع تكراري ترادفي متنوع في الجينوم البشرية في قطاع غزة
Dissertant
Thesis advisor
University
Islamic University
Faculty
Faculty of Science
Department
Department of Biological Science
University Country
Palestine (Gaza Strip)
Degree
Master
Degree Date
2008
English Abstract
Allele frequencies for 15 autosomal Short Tandem Repeat (STR) loci namely Penta E, D18S51, D21S11, TH01, D3S1358, FGA, TPOX, D8S1179, vWA, Penta D, CSF1PO, D16S539, D7S820, D13S317, D5S818 and Amelogenin, were studied in a sample of 125 unrelated individuals from the Palestinian population of Gaza Strip.
PCR amplification was performed with the PowerPlex® 16 PCR amplification system and the amplified products were separated and detected using the ABI 3130 DNA genetic analyzer using reference-sequenced ladder.
No significant departure from Hardy Weinberg Equilibrium (HWE) expectations were observed for 11 of the 15 STR loci analyzed (P-value > 0.05).
The exceptions were the Penta E, D5S818, D13S317 and vWA loci but when the Bonferroni procedure was used as a correction for the multiple tests performed on a population sample, none of the previous P-values could be considered significant.
No linkage disequilibrium was found between the loci located on the same chromosome, and thus they are statistically independent.
The FIS value for Gazan population has been calculated as–0.024.
This low FIS value indicates that the population is in random mating with high level of heterozygosis.
The combined probability of exclusion, power of discrimination, probability of matching value for all the 15 STR loci were 0.999998595; 0.999999986 and 2.73 x 10-18, respectively.
In the light of the obtained results, we can confidently conclude that these 15 markers are suitable for forensic analyses and paternity testing in the Palestinian population of the Gaza Strip.
Main Subjects
Topics
No. of Pages
85
Table of Contents
Table of contents.
Abstract.
Chapter one : Introduction.
Chapter two : Literature review.
Chapter three : Materials and methods.
Chapter four : Results.
Chapter five : Discussion.
Chapter six : Conclusion and recommendations.
References.
American Psychological Association (APA)
Abu Halimah, Masud Said. (2008). Genetic variation of 15 autosomal short tandem repeat (STR) loci in the Palestinian population of Gaza Strip. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-303450
Modern Language Association (MLA)
Abu Halimah, Masud Said. Genetic variation of 15 autosomal short tandem repeat (STR) loci in the Palestinian population of Gaza Strip. (Master's theses Theses and Dissertations Master). Islamic University. (2008).
https://search.emarefa.net/detail/BIM-303450
American Medical Association (AMA)
Abu Halimah, Masud Said. (2008). Genetic variation of 15 autosomal short tandem repeat (STR) loci in the Palestinian population of Gaza Strip. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-303450
Language
English
Data Type
Arab Theses
Record ID
BIM-303450
