Molecular genotyping and antibiogram of staphylococcus aureus isolated from patients admitted to Ramallah Hospital, Palestine

Dissertant

Kaibni, Maha Hanna

Thesis advisor

Isawi, Tamir

Comitee Members

Surur, Mahmud
Farraj, Muhammad A.

University

Birzeit University

Faculty

Faculty of Science

Department

Department Nutrition and Dietetics

University Country

Palestine (West Bank)

Degree

Master

Degree Date

2006

English Abstract

Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are increasingly considered as a main health concern worldwide for hospitalized patients.

The prevalence of community-acquired infections has risen continuously during the last few years.

Therefore, the control of MRSA spread is now more important than ever.

This study was performed to investigate the prevalence of S.

aureus nasal carriage among 843 patients admitted to Ramallah hospital and 72 health care workers (HCW) between October 2003 and October 2004, and to determine phenotypic (antibiogram) and genotypic (Multilocus Restriction Fragment Typing) characteristics of MRSA isolates.

The prevalence of S.

aureus nasal carriage among patients and HCW was 25.8 and 20.8 % respectively.

The prevalence of MRSA isolates among S.

aureus from patients and HCW was 6.8 and 66.6 % respectively.

Eleven antibiogram types were characterized for the 28 MRSA isolates by using 10 different antibiotics.

The most predominant antibiogram was antibiogram I and was observed among 46.4 of all MRSA isolates.

All isolates were completely resistant to penicillin and ampicillin, while all isolates were 100 % susceptible to teicoplanin and vancomycin.

However, the susceptibility to ciprofloxacin, Erythromycin, gentamycin, and clindamycin was 71.5 %, 82.2 %, 85.8 % and 89.3 % respectively.

By using MLRFT method, the 28 MRSA isolates were differentiated into eight restriction fragment types (RFTs).

Twenty-two (78.5 %) of the 28 isolates were grouped into four RFTs.

The remaining six MRSA isolates were assigned to four additional RFTs. Four of the common MRSA RFTs observed in this study could be provisionally identified as belonging to sequence types of known clonal lineages.

Where the RFTs genotypes–CAAACAC, AAACCAA , BBBBBAB and BAAACAC correspond to the archaic / Iberian / clone V group, the New York / Pediatric / Japan group, the epidemic MRSA type 16 (EMRSA–16) group, and the Brazilian clone respectively.

The other RFTs retrieved in this study did not correspond to any of the major lineages that spread internationally.

Main Subjects

Biology

Topics

• Palestine
• Ram Allah
• Genetic aspects

No. of Pages

71

Table of Contents

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-303603